Santa Cruz Biotechnology, Inc.
Occludin Antibody (E-5)
sc-133256
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The aim is to stain Occludin in human gingival epithelial cells using immunofluorescence staining. We could not get positive staining in either frozen sections or paraffin embedded sections.
Immunofluorescence
Human gingival tissue
4C overnight, 1/50-800 dilution
10% goat serum with 1% BSA in PBS
Goat anti mouse Alexa Fluor 488
None
Fluorescence
We could not get positive staining in either frozen sections (acetone or formalin fixation) or paraffin embedded sections (with or without antigen retrieval).
N/A
Nothing
No positive staining, high background
We have tested different ways to try to make it work, but all efforts failed.