BD Biosciences
Purified Hamster Anti-Mouse CD3e Clone 145-2C11 (RUO)
550275
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Our lab studies T cell function both in vitro and in vivo. For in vitro and ex vivo work, we use anti-CD3 + anti-CD28 costimulation for activation or reactivation of CD4+ T cells.
In vivo CD4+ T cell activation
Murine CD4+ T cells
Goat anti-hamster IgG coated plates
None
Anti-CD3 + anti-CD28
ELISA to measure cytokine production
We first coat plates with goat anti-hamster IgG so that the anti-CD3 antibody can bind. We then add cells and anti-CD3 and anti-CD28 antibodies and allow stimulation to occur for 24 hours. Supernatants are collected and measured by ELISA. Robust and reliable activation can be seen when using this method and antibodies.
N/A
Reliable antibody for T cell stimulation
Great antibody for T cell stimulation. Reliable and reproducible results. It is also a relatively inexpensive antibody.