Invitrogen
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568
A-11004
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Our lab routinely performs IHC and IF on tissue sections. For IF, we use secondary antibodies from Invitrogen. They have a long shelf life and give high-quality staining with little background fluorescence.
Immunofluorescence
Mouse brain section
Anti-Olig1
5% milk 5% goat serum
DAPI
Light microscopy, far red channel
Staining with this antibody pair shows decent staining of the target protein. Although there is some background staining, the positive signal is far greater and easily distinguishable making analysis simple.
N/A
Easy to optimize, long shelf life
None
I would recommend this antibody to anyone needing an anti-mouse secondary for IF.