Staining Human Gingival Epithelial Cells

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Research Scientist

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Company:

Invitrogen Antibodies by Thermo Fisher Scientific

Product Name:

Occludin Antibody (OC-3F10)

Catalog Number:

33-1500

The aim is to stain human gingival epithelial cells with this occludin antibody using immunofluorescence. The results are good for both frozen section and paraffin embedded sections.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human gingival tissue

Primary Incubation

4C overnight

Blocking Agent

10% goat serum

Secondary Incubation

Goat anti mouse Alexa Fluor 594

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

Good staining in both frozen and paraffin embedded sections.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works for both frozen and paraffin sections. No antigen retrieval needed for the later.

The Bad

Some background, especially in frozen sections

The Bottom Line

It works for both frozen and paraffin sections.

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