Immunophenotyping in Dogs

Veterinary Diagnostic Laboratory
Kansas State University
RA

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Quality of Results

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Company:

Biolegend

Product Name:

CD11b PE/Dazzle

Catalog Number:

101255

The aim of this experiment is to detect presence of T or B cell lymphoma in dogs. The antibody CD11b is used in gating the granulocytic population in whole blood cells in the dogs. The antibody used worked very well for doing so. Cells positive for CD11b were then used to gate on other granulocytic subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole dog blood in EDTA

Primary Incubation

30mins in dark

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD fortessa

Results Summary

Was able to pick up the granulocytic population from the use of this marker.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

None

The Bottom Line

Will re purchase

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