Etaluma, Inc.
Lumascope 720 Automated Fluorescence Microscope
LS720
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This review is for the Lumascope 720, which has been discontinued and replaced by the Lumascope 850.
Extraction of quantitative data from time-lapse imaging can provide unprecedented insights into cell signaling in single cells. A limiting factor in moving this field forward is the cost of existing live imaging systems, until now. The Lumascope 720 has enabled our small budget laboratory to extract real-time data from our multi-color live cell reporters. Thanks to the 720, we have been able to switch over from expensive ELISA based broken cell assays that measure the average signal across heterogeneous populations of cells at a single time point to now acquiring essentially free data points at single cell resolution over time. Also, thanks to the motorized stage and multiwell format we don't need to use our plate reader anymore.
Live cell quantification of the cell cycle
HT1080 Fibrosarcoma cells
Cells were stably transduced with lentiviral vectors containing nuclear localized red, green, and blue fluorescent protein variants
Let cells equilibrate a few hours after placing on stage to facilitate maintenance of focal plane
Cells were imaged by phase, red, green and blue channels every 5 minutes over 72 hours. Nuclei were detected and image processing was conducted using R and EBImage package.
Bright signals, clear images, easy to use software
N/A
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Everything: super solid manufacturing, bright and clear images, ease of use, cost
Nothing
Great solution for those interested in live cell imaging at an affordable price.