Excellent CD49f Antibody

Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

a-hu CD49f BV421

Catalog Number:

313623

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD326+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers. This antibody was used to identify basal epithelia.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary Human Prostate Cells

Primary Incubation

1:20 25 mins

Blocking Agent

FcX

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

Trimodal staining. Subpopulation of Stroma = negative, Luminal Epithelia = Low, Basal Epithelia = high

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, low background

The Bad

Trimodal population staining

The Bottom Line

Excellent antibody

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