Good IL4 Antibody

Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

a-hu IL4 APC

Catalog Number:

20-7048-T025

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD326+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers. This antibody was used to identify Th2 Cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cultured Human PBMC

Primary Incubation

1:20 25 mins

Blocking Agent

FcX

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

Th2 CD4 T-cells stain positive when cultured with Brefeldin A

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Positives are detectable

The Bad

Not the best separation

The Bottom Line

Need to optimize conditions to get better separation

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