Very Good CD44 Marker

Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

PE-Cyanine7 Anti-Human/Mouse CD44 (IM7)

Catalog Number:

60-0441-U025

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD326+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers. This antibody was used to try and identify putative stem cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary Human Prostate Cells

Primary Incubation

1:20 25 mins

Blocking Agent

FcX

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

Very bright, high non-specific binding by negatives, needs to be titrated down.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very bright

The Bad

Needs to be titrated down a lot

The Bottom Line

Buy but titrate

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