Canine Immunophenotyping

Veterinary Diagnostic Laboratory
Kansas State University
RA

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

CD45RA Antibody (CA4.1D3)

Catalog Number:

NB100-64897

While doing phenotyping of dog cells, this CD45 marker was used to gate over all pan leukocytes. Cells positive in this gate were then used to distinguish other T cell subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole canine blood in EDTA

Primary Incubation

30 mins in dark at 4 degrees

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD fortessa

Results Summary

The antibody was able to identify 90% of all pan leukocytes.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Worked well for staining

The Bad

None

The Bottom Line

Will purchase again

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