Beta Actin Levels In Rat Lung

June 09, 2017

School of Pharmacy
University of Colorado, Denver
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Company:

Invitrogen Antibodies

Product Name:

beta Actin Monoclonal Antibody

Catalog Number:

AM4302

The adult rat lung with and without lung injury was loaded on the SDS-PAGE and blotted for beta actin antibody. It has been found that the levels of the protein does not change with the acute lung injury. The antibody has shown the trend very effectively.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Supernatant of rat lung homogeneate in PBS

Primary Incubation

Overnight at 4 degrees celcius

Blocking Agent

Dry milk powder

Secondary Incubation

2 hr at room temperature

Tertiary Incubation

Not applicable

Detection

Enhanced chemiluminiscence

Results Summary

The antibody shows there are identical levels of beta actin protein in the samples loaded.

DOI or PMID #

Not applicable

Additional Notes

Not applicable

Related Categories

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Summary

The Good

Very specific

The Bad

Needs longer primary incubation with continuous shaking

The Bottom Line

The antibody is very good in identifying endogenous levels of beta actin in rat lung.

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