eBioscience™ from Invitrogen Antibodies by Thermo Fisher Scientific
IL-22 Monoclonal Antibody (IL22JOP), APC, eBioscience™
17-7222-80
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Browse Similar Products: IL-22 Flow Cytometry Antibodies
T cells were cultured in vitro in the presence of IL-6, IL-1b, IL-23, anti-IL-4 and anti-IFNg for 3 days and were then stimulated with Cell Stimulation Cocktail for 4 hrs. Cells were then stained with viability dye, fixed with 2% paraformaldehyde, and stained for intracellular IL-22.
Flow Cytometry
In vitro differentiated Mouse T cells
1:200, 30 mins, 4 C
Mouse Fc block (1:100, 10 mins, 4 C)
None
Red laser
T cells cultured in vitro with IL-6, IL-23, and IL-1b secrete IL-22 protein.
N/A
Works well!
Good option for staining IL-22 on a red laser channel.