PE-Vio770 Anti-CD3 Human

Liver
KCL
Post Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti-CD3-PE-Vio770

Catalog Number:

130-109-463

The goal is to phenotype human blood and liver tissue T cells. Using PBMC isolation followed by flow cytometric cell surface staining for 6mins at 4 degrees with the above Ab. The image shows liver lymphocytes stained for CD3 and iNKT cell marker. Miltenyi Biotec antibodies for me have shown competitive pricing with no loss to quality.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human blood and liver tissue

Primary Incubation

6 minutes at 4 degrees

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

When using this Ab, for over 50 samples now, consistency and clarity is confirmed. I am very happy to use and will contiue to use this Ab.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stable antibody (my student accidentially froze it, but is still working fine!)

The Bad

None

The Bottom Line

Good quality, works, cost effective.

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