Bio-Rad
T100 Thermal Cycler
1861096
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We have two of these thermal cyclers-one was purchased a few years ago, and one was purchased about a year ago. We use these for all of our genotyping and cDNA synthesis and so far they've worked great. We wanted something that was going to last, wasn't terribly expensive, and was easy to use. This cycler has a really user-friendly interface and the touch screen makes it suuuuper simple to input programs. I like that it gives you a countdown to when you'll be done-our older eppendorf cycler doesn't do that!
PCR
RNA/DNA
Protocol depends on what you're genotpying for/what your cDNA synthesis calls for. Very easy to input different programs into the machine and lots of space for different programs.
Remember to save your programs! It doesn't do it automatically
Great consistent results, we've never had an issue with this malfunctioning. Great cDNA and great PCR products. Picture attached is of a gel I ran with products from this cycler.
Touch screen is great!
N/A
Easy to use
Nothing!
Easy, affordable thermal cycler for genotyping and cDNA synthesis.