Good Antibody For Granulocyte Detection

May 26, 2017

Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience from Invitrogen Antibodies

Product Name:

Ly-6G (Gr-1) Monoclonal Antibody (RB6-8C5), PE

Catalog Number:

12-5931-82

The general aim of the research is to study hematopoiesis and acute myeloid leukemia. This antibody was used to identify and isolate granulocytes from murine peripheral blood.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine peripheral blood

Primary Incubation

1:100

Blocking Agent

Fc block is added to cells prior to staining with antibody

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD LSRII-yellow machine

Results Summary

This antibody gives a strong signal allowing the ability to clearly identify Gr1+ myeloid cells in the blood.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong signal

The Bad

None

The Bottom Line

Recommended for detecting and isolating granulocytes from murine peripheral blood

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