Reliable CD45.1 Flow Cytometry Antibody

May 18, 2017

GIVI
The Gladstone Institutes
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

APC Mouse anti-Mouse CD45.1

Catalog Number:

558701

The purpose was to determine the presence of CD45.1 congenic markers vs CD45.2 to prepare for future investigations and breeding.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse PBMCs

Primary Incubation

4 C for 30 minutes at 3:300 dilution

Blocking Agent

FC block (1:1000)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Results successfully detected the presence of CD45.1 markers in our combined samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Available, reliable antibody

The Bad

None

The Bottom Line

Effective and was a successful antibody for this purpose!

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