Detects IL-1 RI Expression In Human Leukemic Cells

May 22, 2017

Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human IL-1 RI PE-conjugated Antibody

Catalog Number:

FAB269P

The general aim of the research is to study cytokine receptor signaling in acute myeloid leukemia. This antibody was used to measure the expression level of IL-1 RI in human AML cell lines.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

TF1 erythroleukemic cell line from human blood

Primary Incubation

1:25

Blocking Agent

Fc block is added to cells prior to staining with antibody

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD LSRII-yellow machine

Results Summary

Expression of IL1RI was detected compared to an isotype control antibody

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong, detectable signal

The Bad

High dilution needed

The Bottom Line

Recommended for flow cytometry

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