Bio-Rad
SsoAdvanced™ Universal SYBR® Green Supermix
1725271
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This is the only SYBR green mix we use with our CFX Connect and we came across it via Bio-Rad's awesome service contract. I don't have a whole lot to compare it to, but it works phenomenally in our hands and I would be very hesitant to trust another green mix with my precious RNA samples. We often run qPCR on smaller populations of sorted cells and I'm sometimes a bit iffy on the amount of RNA I yield, but using the bio-rad system I've always gotten good housekeeping values and believable CTs for my genes of interest. We use it in combination with their cDNA kit too, so I can't really speak to its performance without the full Bio-Rad situation.
qPCR
cDNA
Use it as you would any other SYBR green mix-I use 5ul per well in my 96 well plate (10ul reactions)
Nope
Works great-reliable curves and CT values, easily reproducible using bio-rads full qPCR line.
N/A
Figure is just a handful of melt curves for different genes from a sample plate.
Reproducible, reliable
None
Works well! Would highly recommend if you have a bio-rad qPCR machine-they cut you a deal on the service contract.