Biolegend
Alexa Fluor® 700 anti-mouse CD4 Antibody
100536
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Browse Similar Products: CD4 Flow Cytometry Antibodies
Our lab studies the T cell populations in mice before and after an influenza infection. We are interested in how the different T cell populations change and therefore need a good CD4+ T cell marker. We chose this one because it is relatively dim and the CD4+ is very large.
Flow Cytometry
Mouse lung cells
Cells were stained in 100uL FACS buffer with antibody at 1:200; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
BD Fortessa
This antibody works well and easily shows the separation between CD4+ and CD8+ T cells in our whole cell sample.
N/A
Dim, good for large populations
This antibody works well for mouse CD4+ T cell population identification.