The Antibody Stains NK1.1+ Cells Very Well

May 04, 2017

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cy5 anti-mouse NK-1.1 Antibody

Catalog Number:

108716

We wanted to look at NK cells in healthy vs diseased states, so we used this antibody to identify NK cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse splenic cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody clearly stains NK1.1+ cells well

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains NK1.1+ cells well

The Bad

None to mention

The Bottom Line

The antibody is great for identifying NK cells

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