Antibody Stains MHC II+ Cells In The Spleen Very Well

April 28, 2017

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Alexa Fluor® 647 anti-mouse I-A/I-E Antibody

Catalog Number:

107618

We wanted to study splenic cells in the disease vs healthy state, so we wanted to us this antibody to identify a number of cell populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse splenic cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody stained MHC II+ cells very clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains MHC II+ cells very well

The Bad

None to mention

The Bottom Line

Great antibody for identifying MHC II+ cells

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