Anti-Mouse TLR4

May 02, 2017

Biology
Abbvie
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies by Thermo Fisher Scientific

Product Name:

anti-mouse TLR4 Biotin

Catalog Number:

13-9924-81

The purpose was to determine basal level of expression of TLR4 in the naïve BALB/c mouse spleen. This antibody was the only one available under this fluorochrome.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

0.25ug per test 30 minutes on ice

Blocking Agent

Fc block 5 min on ice

Secondary Incubation

0.015ug streptavidin conjugated BV421 antibody 15 minutes on ice

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

The results show very low expression of TLR4 on myeloid cells in the spleen. This is expected in the naïve animal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Need a secondary reagent

The Bottom Line

Good antibody for the purpose

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