ZYMO Fecal DNA Extraction Kit

Mitchell Cancer Institute-research wing room 2095
reasearch-proteomics
PhD. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Zymo Research

Product Name:

ZR Fecal DNA MiniPrep™

Catalog Number:

D6010

After battling with several DNA extraction kits using a complex sample I found that this kit destroyed the competition with yield, speed and ease of processing, and cost. This prep was essential for me to perform high throughput sample processing with extremely time sensitive clinical samples. The yield was 10 fold above the competition. It performed perfectly for all of my downstream analysis. This does require a bead beater, For high throughput sample preparation, I would suggest one that holds multiple tubes. Also, do not forget to spin the IV-HRC tubes PRIOR to adding the sample. I made this silly mistake one time. The DNA yield and quality still remained higher than competitor kits, just more dilute when compared against the samples prepared properly using the sun down IV-HRC tube.

Experimental Design and Results Summary

Application

DNA Isolation

Starting Material

Human gastrointestinal lavage

Protocol Overview

The kit was used to isolate 16S from human samples for downstream analysis of sequencing.

Tips

Spin IV-HRC column material out of tube before adding sample.

Results Summary

10 fold greater yield than competition, great down stream results, extremely efficient and high-throughput.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Quality results, extremely efficient, extremely consistent

The Bad

Annoying to unscrew and snap tips off of tubes, but makes up for it in overall processing time

The Bottom Line

I would recommend this product to anyone who values time, quality, and consistency. This product was a life saver compared to the competition and was essential to saving critical clinical samples.

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