Fas Expression On B Cells

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Fas (CD95) PE-Cy7

Catalog Number:

557653

The aim of the research was to determine the expression of Fas on splenic B cells 7 days after immunization with sheep red blood cells. The antibody was chosen because it fit our panel well and there were other options with other fluorophores.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1: 100, 4 degrees, 30 min

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Clear separation of Fas+ cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, clearly marks positive cells

The Bad

None

The Bottom Line

Good antibody for measuring Fas expression.

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