MojoSort™ Mouse CD8 T Cell Isolation Kit Review

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

MojoSort™ Mouse CD8 T Cell Isolation Kit

Catalog Number:

480007

Our lab has generated a mouse model that expresses three transgenic human host factors that allows successful acute HIV infection in these mice. We are currently testing a novel vaccine candidate, and we are examining CD8 T cell responses in peripheral blood and spleen upon antigenic stimulation in samples from immunized mice. Cells are purified from mouse spleens and stimulated with antigen in vitro. In order to avoid activation by positive selection, we looked for a negative selection solution.

Experimental Design and Results Summary

Application

Cell purification

Starting Material

Mouse spleen

Protocol Overview

Follow the instructions included

Tips

Nothing in particular

Results Summary

Selection works well. We get >95% selection. I have not used other negative selection kits, so I'm unable to compare to other products.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good separation; reasonable price

The Bad

None

The Bottom Line

I recommend it.

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