CD158e/k (KIR3DL1/DL2)-PE-Vio770 Antibody Review

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD158e/k (KIR3DL1/DL2)-PE-Vio770, human

Catalog Number:

130-099-958

Our lab is interested in the protective role of NK cells towards acute HIV infection. We have developed in vitro assays to study their role in killing HIV infected cells, and we examine their cytokine and KIR receptor expression via flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Purified NK cells in culture with target cells

Primary Incubation

1:10 for 30 min at 4 degrees Celcius

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Good detection of KIRs

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

Need to use it at high concentration

The Bottom Line

Good antibody

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