Miltenyi Biotec
FcR Blocking Reagent, mouse
130-092-575
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One of the models that we have generated expresses three transgenic human host factors that allows successful acute HIV infection in these mice. We isolate different cell populations to study vaccine-induced immune responses and perform detailed FACS analysis. Some of these populations, such as NK cells and monocytes have high FcR expression, so this blocking reagent is helpful to block unspecific staining.
Flow Cytometry
Mouse splenocytes and peripheral blood
1:100 for 30 min at 4 degrees Celcius
This is a blocking reagent
Other antibody of interest, such as CD14
None
Flow cytometry
We have a cleaner and more specific FACs staining when using this product.
N/A
Good blocking reagent
I recommend it for cells with high FcR expression