Anti-Mouse CD3 FITC

April 19, 2017

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

Anti-Mouse CD3 FITC

Catalog Number:

11-0032-82

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Cells

Primary Incubation

1 hour on ice with surface stain antibodies

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

None

Tertiary Incubation

NA

Detection

FACS

Results Summary

CD3 FITC used to make Lineage negative selection cocktail.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Good antibody for negative lineage selection

The Bad

None

The Bottom Line

Good antibody for negative selection of lineage positive murine cells.

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