R&D Systems
Mouse IL-1 beta /IL-1F2 PE-conjugated Antibody
IC4013P
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.
Flow Cytometry
Murine Cells
1 hour on ice with surface stain antibodies
Blocking with 2% BSA and Fc block (1:100)
1:50 dilution on ice with intracellular staining antibodies
NA
FACS
Significant intracellular IL-1 beta levels were detected.
Good flow cytometry antibody for IL-1 beta intracellular staining
None
Good antibody for IL-1 beta intracellular staining murine T cells.