Invitrogen by Thermo Fisher Scientific
anti-Calnexin antibody
MA3027
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This antibody was used to perform confocal microscopy on HeLa cells. Cells were also transfected with an ER specific fluorescent protein (addgene plasmid #56309). The antibody showed very strong staining, but with a pattern that is more consistent with mitochondria than endoplasmic reticulum.
Immunofluorescence
HeLa Cells
1 hour room temperature at 1:300 in blocking agent
PBS with 1% normal goat serum and 0.1% triton-x 100
Goat anti-mouse conjugated to Alexa 568 for 1 hour room temp at 1:300
ER-targeted mClover transfection
Confocal microscopy
The antibody appears more specific to mitochondria than endoplasmic reticulum. Although the ER and mitochondria are intimately associated there is little overlap with the positive control: a fluorescent protein (mClover) tagged cytochrome p450 expression construct. Follow-up studies with mitotracker show strong colocalization. I would not use this antibody if you are specifically looking to target the endoplasmic reticulum.
N/A
Strong reactivity for mitochondria
Not specific for the endoplasmic reticulum
I would use an alternative antibody if you are looking for an ER-specific stain.