CD8a Brilliant Violet 510

April 06, 2017

Research
Bloodworks NW
Postdoctoral research fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 510™ anti-mouse CD8a Antibody

Catalog Number:

100752

This clone of anti-CD8a antibody is commonly used in immunology labs to detect mouse CD8+ T cells. We chose this fluorochrome (BV 510) in our efforts to design a 10 color panel to detect CD8+ and CD4+ T cells in the spleen and thymus.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

30 mins @+4c

Blocking Agent

anti-cd16/21

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

Shown here a initial titration experiment with 2-fold dilutions between 1:50 to 1:800 to determine the optimal amount needed to stain 2 million cells. Based on calculation of signal to noise ratio and the histogram plots, we decided to use 1:200 for all our subsequent experiments.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Excellent separation between positive and negative populations. Low background staining

The Bad

Significant overflow into the neighboring BV605/SB600 channel.

The Bottom Line

Excellent choice for the BV510 fluorochrome. When designing staining panel, ensure that antibody used in BV605/SB600 channel is not co-expressed on CD8+ T cells to minimize the impact of the significant spillover from BV510.

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