Anti-Mouse IgG2b Alexa Fluor 568 Secondary Antibody For IF And IHC

Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies by Thermo Fisher Scientific

Product Name:

Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

Catalog Number:

A-21144

Our lab studies the role of CD4+ T cells in inflammatory processes both in vitro and in various in vivo models. One model we use is experimental autoimmune encephalomyelitis (EAE). We often perform IHC on CNS and peripheral immune tissues to analyze various cell populations.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse CNS tissue sections

Primary Incubation

anti-CC1/APC mouse monoclonal antibody at 1:20

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

Tertiary Incubation

DAPI

Detection

Fluorescent microscope

Results Summary

The secondary antibody used specifically stains desired target with little to no background. The concentration of secondary antibody used is very low which allows for the use of this antibody for an extended period of time.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Little antibody is needed

The Bad

None

The Bottom Line

If performing IF and/or IHC, this is a great secondary antibody to use against murine primaries.

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