Real-Time PCR Machine

University of Maryland, College Park
Cell Biology and Molecular Genetics
Post-doctoral researcher

Overall

Performance

Ease-of-Optimization

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Company:

Thermo Fisher Scientific/Applied Biosystems

Product Name:

StepOnePlus™ Real-Time PCR System

Catalog Number:

4376600

Many of the real-time PCR experiments done in our lab use SYBR Green. The StepOnePlus machine has delivered very good results with little variability between plates.

Experimental Design and Results Summary

Application

Real-time PCR

Starting Material

cDNA

Protocol Overview

Put loaded PCR plate onto machine, set up plate layout and thermal cycling parameters on machine, start plate

Tips

Optimize primer annealing temperatures before running many samples

Results Summary

Ample amplification with little variability between plates.

Features Summary

Included software can before fold-change analysis

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Small footprint

The Bad

Cost

The Bottom Line

Great PCR machine with small footprint that delivers reliable results

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