Abcam
Anti-Phospholipase A2 antibody (ab23705)
ab23705
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Browse Similar Products: Phospholipase A2 Immunohistochemistry Antibodies
Our lab is interested in PLA2-IIa and its role as a anti-bacterial reagent. We wanted to test its levels in the oral epithelium (keratinocytes). We chose this antibody because it has been published by Pernet E et al. Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity. Nat Commun 5:5105 (2014).
Immunohistochemistry
Gingival tissue from rhesus monkeys
1:1500 dilution. 45 minutes incubation at room temperature
3% H2O2 and ACUITYAdvanced Reagent 1 (Serum Block) from BioLegend
ACUITYAdvanced Reagent 2 (Boost) from BioLegend
ACUITYAdvanced Reagent 3 (HRP Polymer) from BioLegend
DAB
We saw a good signal in the basal layer of the epithelium (weak to moderate), and a stronger positive staining in the inflammatory cells in the subcutaneous tissue.
N/A
Short incubation, low concentration needed
Is on the expensive side
A good antibody that stained our FFPE sections well