Novus Biologicals
RAMP2 Antibody (4E5)
NBP2-01853
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Browse Similar Products: RAMP2 Immunohistochemistry Antibodies
We are interested in assessing the levels of certain neuropeptides and their receptors in epithelial tissue. We chose this antibody because the images on Novus's website looked convincing (in human kidney sections). It also has been verified for IHC on FFPE tissue.
Immunohistochemistry
Human gingival tissue (epithelium)
Dilution: 1 in 100. Overnight incubation, at 4°C.
in 3% hydrogen peroxide followed by ACUITYAdvanced Reagent 1 (Serum Block)
ACUITYAdvanced Reagent 2 (Boost)
ACUITYAdvanced Reagent 3 (HRP Polymer)
DAB (3,3'-diaminobenzidine)
We detected membranous and cytoplasmic staining in the keratinocytes. This is the pattern of expression that was predicted, and is biologically meaningful. We also saw some staining in stromal inflammatory cells, which is also not unexpected.
N/A
Specific staining - reproducible data
Need to use it at a fairly high concentration
A good antibody that performed well at the first test!