Anti DsRed Antibody Can Detect TdTomato

March 27, 2017

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Purified Mouse Anti-DsRed with Control

Catalog Number:

551814

U2OS cells were transfected with p-tdTomoto-N1, and could be deteted by Western blot analysis using anti-DsRed

Experimental Design and Results Summary

Applications

Western Blot

Sample

Transfected U2OS lysate

Primary Incubation

1:2000, overnight

Blocking Agent

5% milk

Secondary Incubation

1:5000, 1 hour

Tertiary Incubation

NA

Detection

ChemiDoc™ Touch Imaging System

Results Summary

Clean band at ~ 70 kDa was detected

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Easy to perform

The Bad

Relatively high background.

The Bottom Line

Can detect tdtomato

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