Abcam
Anti-p38 (phospho Y182) antibody
ab47363
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Browse Similar Products: p38 Western Blot Antibodies
The general aim of my research is to study signaling pathways involved in the pathogenesis of leukemia. The specific goal of the experiment for which I used the product was to determine whether leukemia cells respond to IL-1 stimulation. The reason I selected this product is because I have had previous success with Cell Signaling phospho-antibodies.
Western Blot
THP1 human leukemic cell line from blood
1:1000, overnight at 4 degrees C with rocking
TBS + 0.1% Tween + 5% w/v BSA
1:5000 anti-rabbit-HRP, 1 hour at room temperature with rocking
None
ECL
strong band close to 40 kDa, increases in strength upon IL-1 stimulation for 15 minutes
One clear band
I recommend this antibody for p-p38 detection