IgG Isotype Control

April 03, 2017

Veterinary Diagnostic Laboratory
Kansas State University
RA

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Rabbit Polyclonal IgG Isotype Control

Catalog Number:

NBP2-24892

This isotype control was used as a negative control to distinguish red blood cells bound to IgG in case of dogs. For trying out the antibody for the 1st type it met all expectations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood in EDTA in dogs

Primary Incubation

20 mins in dark at 4 degrees

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS CAlibur

Results Summary

Worked well as a negative control and was able to separate negative or unstained population,

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well and meets expectation

The Bad

N/A

The Bottom Line

Will use again.

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