Great SYBR Green Reagent for Real-time PCR

Albert Einstein College of Medicine
Pathology
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BIO-RAD

Product Name:

iTaq™ Universal SYBR® Green Supermix

Catalog Number:

1725120

Our lab uses SYBR Green as our real-time PCR platform. This reagent provides reliable and reproducible results with great amplification and little background noise.

Experimental Design and Results Summary

Application

Real-time PCR

Starting Material

cDNA

Protocol Overview

Generate cDNA, Make master mix with optimal primer concentrations, add master mix to real time PCR plate, add cDNA, seal plate, run plate and collect data

Tips

Optimize annealing temperature for primers, perform melt curve analysis

Results Summary

Repeatable, reliable results with little background noise and optimal amplification

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to set up PCR experiments with great results

The Bad

The price may not be as low as other competitors

The Bottom Line

Great SYBR Green reagent for RT-PCR experiments

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