Akt3 Mouse mAb For Western Blot

Pathology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Akt3 (L47B1) Mouse mAb

Catalog Number:

8018

Our lab focuses on proteins in the CNS and their role in neuronal protection and neuroinflammation. We wanted to assess the levels of our protein of interest in CNS tissue in mice under different experimental conditions.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse homogenates from Multiple Sclerosis lesion homogenates

Primary Incubation

Akt3 (L47B1) Mouse mAb

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

LI-COR anti-mouse IRDye secondary antibody

Tertiary Incubation

None

Detection

LI-COR Odyssey

Results Summary

The antibody stains blots with a single band at the correct molecular weight.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Single band at the correct molecular weight

The Bad

Only good for western blot

The Bottom Line

Great antibody for western blots with reactivity across multiple species.

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