Bio-Rad
Goat anti Mouse Interleukin-12
AAM33
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.
Western Blot
Mouse tissue homogenates
0.2 ug/mL antibody, in 5% BSA, overnight at 4 degree
5% skimmed milk in 1X TBST
1:2500 at room temperature for 2 hour
NA
Western Blotting
Decent IL-12 signal was detected from mouse tissue homogenates.
Good primary antibody, no background
None
Efficient detection of IL-12, with minimal background.