IL-9 Antibody

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

IL-9 Antibody [Unconjugated]

Catalog Number:

AF409

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue homogenates

Primary Incubation

0.1 ug/mL antibody, in 5% BSA, overnight at 4 degree

Blocking Agent

5% skimmed milk in 1X TBST

Secondary Incubation

1:2500 at room temperature for 2 hour

Tertiary Incubation

NA

Detection

Western Blotting

Results Summary

IL-9 was detected in mouse tissue homogenates.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Good signal with primary antibody

The Bad

None

The Bottom Line

Efficient detection of IL-9, with minimal background.

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