Abcam
Anti-Paxillin antibody [Y113]
ab32084
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We stained PFA fixed U2OS cells to localize the focal adhesions. We utilized this antibody to compare focal adhesion size in migrating cells.
Immunofluorescence
PFA Fixed U2OS cells
1:500 at room temperature for 1.5 hours
5% NGS
1:750 rhomadine conjugated anti-rabbit antibody
None
Confocal Microscope
We were able to localize the paxillin protein (shown in green) to the focal adhesions and to compare focal adhesion sizes. This antibody gives a very clean signal so is ideal for such studies.
N/A
Clear binding of antibody.
None. Clean.
Excellent rabbit monoclonal antibody to paxillin for immunofluorescence. Would highly recommend.