LI-COR Secondary Antibody For Detection In The 680 Channel

Pathology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

LI-COR

Product Name:

IRDye® 680LT Goat anti-Rabbit IgG (H + L)

Catalog Number:

925-68021

The LI-COR blot scanning systems are a great alternative to traditional dark room methods for developing western blots. The secondary antibodies can be used to multiplex blots without stripping and reblotting, granted your primaries are a different species and/or expected band sizes are separated enough to distinguish.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse brain homogenate

Primary Incubation

Anti-GAPDH

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

IRDye® 680LT Goat anti-Rabbit IgG (H + L)

Tertiary Incubation

None

Detection

LI-COR Odyssey

Results Summary

Clear bands with great signal.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Can be used at a low dilution and multiple proteins can be visualized in different channels without stripping

The Bad

None

The Bottom Line

Great antibodies if you're using the LI-COR systems

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