High Fidelity PCR Mastermix For Conventional PCR Applications

Albert Einstein College of Medicine
Pathology
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific/Invitrogen

Product Name:

Platinum® PCR SuperMix High Fidelity

Catalog Number:

12532016

We use this supermix for in-house mouse genotyping. The PCR mix is ideal for anyone performing conventional PCR amplification with subsequent gel electrophoretic analysis.

Experimental Design and Results Summary

Application

PCR

Starting Material

Mouse DNA extracts

Protocol Overview

Extract DNA from starting material, prepare PCR master mix by combining Supermix and primers, load master mix into PCR vessel, add DNA, perform PCR

Tips

Determine optimal primer concentrations and Tm for PCR assay

Results Summary

Great PCR amplification from starting DNA material

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Widely applicable to many conventional PCR applications.

The Bad

Somewhat pricier than other products

The Bottom Line

Good supermix for conventional PCR applications.

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