Great Anti-Mouse Secondary Antibody In 800 Channel For LI-COR Western Blot Scanner

March 15, 2017

Pathology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

LI-COR

Product Name:

IRDye® 800CW Goat anti-Mouse IgG (H + L)

Catalog Number:

925-32210

Browse Similar Products: IgG Western Blot Antibodies

The LI-COR western blotting machines have been a great addition to our lab for western blot visualization. The secondary antibodies offered are high quality and give great signal with even the weakest bands/low abundant proteins.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue homogenate

Primary Incubation

Mouse Anti-Akt3 at 1:1000

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

IRDye® 800CW Goat anti-Mouse IgG (H + L) at 1:25,000

Tertiary Incubation

None

Detection

LI-COR Odyssey

Results Summary

This secondary antibody offers detection in the 800 channel on the LI-COR Odyssey or any other LI-COR scanner. Although the fluorochrome conjugated to this secondary does present with some background signal, it is low enough to be manageable and still allows high quality data to be obtained.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Low dilution needed for staining

The Bad

Some background is seen with the 800CW secondaries

The Bottom Line

If you're using the LI-COR scanners, this is a great antibody to use for detection in the 800 channel if you're blotting with more than one antibody.

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