Kit For Isolation Of RNA From Cells

Albert Einstein College of Medicine
Microbiology & Immunology
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

RNeasy Plus Mini Kit

Catalog Number:

74134

Our lab studies different aspects of HIV infections. One of the experiments done is to use different latency reversal agents to reactivate latent HIV from patient cells. To detect reactivated virus we isolate RNA from cells to quantify viral load. We use this kit for RNA isolation.

Experimental Design and Results Summary

Application

RNA isolation

Starting Material

Human PBMCs

Protocol Overview

Follow the Qiagen protocol.

Tips

Can use RNAlater reagent to stabilize the RNA

Results Summary

We consistently get high yields or high purity RNA

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Consistent, easy to use

The Bad

None

The Bottom Line

Would recommend

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