Kit For Isolation Of Viral RNA From Plasma

Albert Einstein College of Medicine
Microbiology & Immunology
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

QIAamp MinElute Virus Spin Kit

Catalog Number:

57704

Our lab works with different mouse models to study different aspects of HIV infection. We use this kit to isolate viral RNA from mouse plasma. We use the RNA isolated with this kit in RT-PCR to detect HIV viral load in these mice following different types of treatment.

Experimental Design and Results Summary

Application

RNA purification

Starting Material

Mouse plasma

Protocol Overview

Follow the detailed Qiagen protocol, very straightforward

Tips

None

Results Summary

consistently get high yields and purity of RNA from mouse plasma. We prefer this kit to the "Viral RNA mini kit" because it allows us to elute the RNA in a smaller volume and therefore gives us higher RNA concentration.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Consistent, easy to use

The Bad

None

The Bottom Line

We routinely use this kit in lab with consistent results. Would recommend.

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