Anti Human CD5 Antibody

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

APC Mouse Anti-Human CD5

Catalog Number:

555355

One of the assays used in our lad is an in vitro ADCC assay using activated ACH2 cells as target cells and human NK cells as effector cells. We use the anti CD5 antibody in order to distinguish between the target and receptor cells, as ACH2 cells express the CD5 marker.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Co-culture of ACH2 cells and human NK cells

Primary Incubation

1:100, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

We get a clean population of CD5 positive cells when staining with this antibody

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

None

The Bottom Line

Works well, would recommend

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