Clean And Reliable Staining Of CD11c

March 14, 2017

Department of Pediatrics
University of California, San Diego
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBiosciences

Product Name:

Anti-Mouse CD11c PerCP-Cyanine5.5

Catalog Number:

45-0114-82

Murine lungs analyzed by flow cytometry and stained in 8 color Ab panel. CD11c staining is clear and reliable

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lung

Primary Incubation

1:200 4C 30 min

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

PerCP-Cy5.5

Results Summary

Clean staining as analyzed by flow cytometry. No significant issues with compensation, stain looked good on fixed and unfixed cells (couldn't notice any difference between the two). Easy detection of alveolar macrophages and dendritic cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy, reliable staining of CD11c

The Bad

N/A

The Bottom Line

Happy with purchase and results, would buy again.

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